One method is enzymatic and the other method is based on immunochemistry
You just have to compare the enzymatic activity and the immunologic activity of your protein based on the physical presence of your protein (based on the absorbance at 280 nm) or relative activity of your protein per unit cited in other papers (if your protein is not purified).
Comparison of two methods, and demonstrate that the new method is equivalent to the reference must contain the critical elements of validation of reference method (linearity standards, coefficient of variation, Horwitz equation, LOD, LOQ, reproducibility and repeatability). Tested parameters must be below or at the same level parameter reference method. Of course refers to all the analytes are measured.
Plot results for the enzyme assay against results for the immuno-assay. Do linear regression and calculate correlation coefficient (R). The nearer R is to 1 the better the correlation between the two assays.
Thank you professor. and can i compare two assay by theirs sensitivity, reproducibility and specificity ?
those parameters you calculate for each assay. Compare then in a table. Try to select the assay giving best sensitivity, reproducibility and specificity. Cost may also be a factor as well as ease of assay.
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