Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

How can I add the metal oxide NP's to the cell lines? I have to make some drug and NP conjuhgates. How much concentration to use? Need some help?

Hi everyone. I am working on metal oxides. I need to make some conjugates of drugs and NP. But I am unsure of what concentrations to make. I have read some articles, but they haven't mentioned...

10 February 2021 3,372 2 View

Can I use a time series dependent variable in a panel regression?

Hi all Can I run a Gmm model with a time series dependent variable? I have a sample of 12 countries and years from 2000 to 2018

03 February 2021 6,491 6 View

the drug loaded nanoparticles?

Hi, I am new to this cancer cell lines field. My research is using the drug loaded nanoparticles and their effects on cancer cell lines. have a question regarding the drug loading. I have...

24 January 2021 8,258 3 View

How to calculate an endotoxin limit with a drug concentration in vp/mL?

Hello, I am trying to validate an endotoxin method for an excipient. I need to calculate my endotoxin limit. My excipient is 0.1% final product and the drug concentration is expressed in vp/mL....

20 January 2021 1,262 1 View

Is it possible to determine nitrogen concentration colorimetrically, using spectrophotometer in macroalgal tissue?

I'm trying to estimate total nitrogen content in seaweeds without using the Kjeldahl method. Thanks in advance

20 December 2020 9,190 2 View

Why is my passive reference dye (ROX) decreasing in fluorescence as my target (FAM) increases during qPCR?

I am running qPCR on eDNA samples from rivers and have found that the vast majority of my samples show a decrease in fluorescence in my passive reference dye (ROX) while my target dye (FAM)...

15 December 2020 723 4 View

Is it possible to trap air and convert it to plasma?

How can I trap air and then convert the trapped air into plasma?

06 December 2020 624 7 View

How to deal with missing data for Reliability Analysis?

Hi, I have a dataset with missing values. I want to do a reliability analysis with SPSS and then implement the data in Mplus. Mplus can handle missing data due to the FIML. I am unsure how to...

21 November 2020 4,658 3 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

Does anyone knows about a comercial software to record pressure and temperature during reaction in an Autoclave?

Dear all: Currently I am working with polymerization reaction in a reactor. For this, I need to control temperature, pressure, agitation speed etc. For calculation of rate of polymerization, I...

07 February 2021 355 2 View

What makes yellow color appear in phosphorus sample after autoclave digestion?

I was using autoclave for digesting water samples for Total phosphorus measurement with ascorbic acid method. The autoclave was set to perform 2 hours digestion and around 121 Celsius degrees. I...

25 January 2021 206 3 View

Disposing Congo Red Agar?

Hi all, Hope you are well. My lab is planning to use some Congo red agar (0.01%) for Shigella flexneri, and we are wondering how do you dispose of the used agar plates. The Shigella contaminated...

13 January 2021 9,823 2 View

How can Sea water impact the hydrodynamic size of Nanoparticles differently than the same Sea water if it is autoclaved?

I am using the same sea water as autoclaved as well as non-autoclaved after adding the nanoparticle. I am getting different Hydrodynamic size for the same nanoparticle.

06 January 2021 3,184 3 View

Please share some research papers on the synthesis of Mn3O4 nanoparticles via Hydrothermal method by using Manganese(II) nitrate hydrate?

05 January 2021 9,040 4 View