I'm working on halophytes, "salt loving" plants, that thrive and survice wild in natural salty soils. These plants, in opposite to sensitive species, like salt and use it as nutrients for their growth and survival. So they accumulate it in their leaves and stems without causing toxicity. I would like to try applying EIS as a quick and non destructive methods to follow the movements of salt ions through plant cells.
Hi Karim, surely EIS is a technique that can give you nice results and a new approach to the study of salt transport in halophytes. The only caveat is about the interepretation of the data, as EIS normally require a littel bit of experience to be used efficiently. Anyway, feel free to contact me at my email in any moment for further discussion
Hi Karim,
thank you for your question.
I must admit I do not know publications applying IES on plant tissues, though there are several papers describing results found with animal tissues.
I think interpretation of data will be difficult, because there will be a lot of reactions interfering with the IES signals. One important aspect will be to keep the turgor constant and to monitor transpiration (as you plan to use intact plants).
I would start with short time experiements (minutes to hours).
Did you solve the problem of attaching the electrodes to the plant surface without interfering with ion distribution in plant tissues?
Good luck!
Best wishes,
Bernhard
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