VIF very high, but correlation is low and coefficient is significant. Which one whould I choose?

Hello. I have a question whether if my coefficients (statistically significant) would be valid when VIFs are really high. In my model, there are independent variables(IV), moderating variables...

26 February 2021 956 13 View

How to clean up TEM grid with plasma cleaner?

I need to clean the hydrocarbon contamination on the carbon substrate of TEM grid. However, I heard that substrate could be broken by the powerful plasma. My substrate is pure carbon, with ~3nm,...

23 February 2021 3,262 5 View

Sb2Te3 thin film annealing and damage(break)?

21 January 2021 9,642 2 View

When comparing two very similar surveys taken over time, but with different sample size, what would be the best way to approach this problem?

Hi, I'm currently analysing a survey for a university course design, and some guidance would be very much appreciated. The students who are enrolled in a (year-long) course were given a link to...

08 December 2020 6,624 9 View

When analysing survey using Likert scale, is it okay to use Friedman's test?

Hi, I'm currently analysing a survey for a university course design, and some guidance would be very much appreciated. The students who are enrolled in a course were given a link to participate...

06 December 2020 6,060 2 View

Which time-serie model is good when using concatenate data?

I would like to make a model using sequence datasets. As I've searched that , many LSTM, RNN tutorials use long-sequential data such as weather in 1950-2020. However, my datasets are short...

24 November 2020 808 3 View

Could you answer some basic questions on CRISPR-cas9?

Hi, I would like to ask simple some questions related to CRISPR-Cas9 that I could not understand by reading some basic articles on it. I am not in that scientific field so my questions might be...

21 October 2020 2,439 2 View

How can I design resin column for test?

I'm interested in the research field of ion exchange resin. I want design about resin column with acryl for test. How do you usually design the diameter and length of small columns for test? I...

13 October 2020 3,443 2 View

How do you check the locations of brain-implanted cannulas?

Hello. I implanted single cannulas bilaterally into the brains of mice to infuse drugs. After behavior tests, what would be the simplese way to validate if the cannulas are in the target areas?...

18 September 2020 8,457 3 View

Why this HPLC data show negative peak?

Lysine was dissolved in mobile phase and injected in HPLC but there are two peaks. One for positive at 2.1 min and another for negative at 2.8 min? Contents of moilbe phase are ACN:Water:TFA =...

08 July 2020 2,695 13 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

How can I find a Raman spectrum wavenumber corresponding to what compound?

I have several Raman spectra with very distinct peaks at certain wavenumbers, such as 277, 359, 466, 583, 623, 686, and 795cm-1. How can I identify them to what compound(s)? The RRUFF and others...

24 February 2021 363 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View