I refilled my lab's 0-7% Baracharte (IDK how to spell this) CO2 meter with Fyrite fluid meant for 0-20%. I didn't know until after the fact. I had no idea there were two different ones and can't find any information on how they might differ from one another. Does anyone know if this would be ok for the device? And how might this impact the CO2 reading?
We do have two 0-20% meters but their lids are kinda stuck because of the white precipitate. Also, these fluids are so expensive so I am hoping to get away what I've worked with so far. 😬
If it is for monitoring CO2 in cell culture incubators .... modern incubators have CO2 sensors (INFRA RED) much more precise than the fyrite hand apparatus I would be more confident with the incubator than with the fyrite stuff...
I have refilled numerous Fyrites over the years and always used 0-20% solution. However, I believe the solution would work in a Fyrite that is graded from 0-7%. The only issue i can see is that if your incubator has for example 8% CO2, which is required for suspension HEK cells, then your Fyrite could not measure this level accurately. Concerning an earlier comment, I would never rely on the CO2 level which is indicated by the incubator without first calibrating the reading first using a Fyrite. I have seen many times incubators indicating 5% CO2 which after testing with a Fyrite turned out to be way less or way more. When cells start growing strangely in a CO2 incubator, the first thing I do when troubleshooting is to measure the CO2 level with a fyrite.
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