Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Handheld Portable Raman spectroscopy systems - recommendations and experience?

Hi all, I am looking for some general feedback and information from those that have experience using handheld/portable systems? I am looking at the prospects of using an off the shelf system from...

07 February 2021 9,691 3 View

How to reverse unit direction of odds ratios for meta-analysis?

Dear all, I am trying to figure out how to convert odds ratio per unit decrease into beta value per unit increase. E.g. OR = 2.3 per 1 mg/dL decrease -> Beta = x per 1 mg/dL increase. I am...

02 December 2020 7,467 3 View

How to prevent protein aggregation in gel filtration (size exclusion) column ?

I am currently trying to run solubilised mitochondria extracts from mammalian cells through a gel filtration column in order to separate proteins by size & visualise protein complexes. My...

29 November 2020 1,483 5 View

How to conduct Hayes Process when serial and parallel mediation is present?

For my study I conducted, the model displayed below is used. It contains parallel and serial mediation. How do I get the results using Hayes process? I feel like I can only conduct parallel or...

28 November 2020 3,935 1 View

GAMM time course analysis: Can you explain why a sig difference is indicated via Parametric coefficients, but smooth term does not improve model fit?

Hello, I have a couple of years experience with GAMMs and I have run several complex models that have shown some really nice time course effects - well controlled structures too. In a new study,...

02 November 2020 9,810 1 View

How to interpret the quality level of animal studies by using CAMARADES tool?

We have been working in a systematic review of animal studies and are performing risk of bias and quality assessment using SYRCLE and CAMARADES tools, respectively. Thereby, we would like to...

26 October 2020 7,171 1 View

Am I introducing any potential loss of RNA integrity by combining an RNALater solution with a non-ionic detergent?

I am planning to characterise the transcriptome of pre-anthesis kiwifruit anthers. The samples are being harvested on my behalf and sent down by post, and I cannot ask the harvesters to dissect...

10 October 2020 1,830 3 View

How can one automatically extract drainage basin values from a DEM following Strahler stream ordering approach?

Analysing hydrologically corrected DEM avails an opportunity to calculate morphometric indices such as drainage basin density(DD) (which takes manual approach). Can not DD and others be extracted...

07 October 2020 3,893 3 View

Is it possible to calculate allelic richness without a genotype matrix?

I am doing a meta-analysis on the impact of humans on fragmenting freshwater ecosystems. I have collected locus-level data above and below dams to assess how expected heterozygosity (He), observed...

14 August 2020 4,255 1 View

How do you calculate a discrimination index for polytomous data?

Hello, I'm currently running a PCA on a polytomous data set (5 point Likert). I want to create a discrimination index to narrow down my items before running the PCA. After looking around, I'm...

13 February 2021 3,517 2 View

SmartPLS: What should I do if discriminant validity HTMT>0.90?

Dear researchers, I have HTMT larger than 0.9, and I have read the article by Henseler et al (2015) :A new criterion for assessing discriminant validity in variance-based structural equation...

19 January 2021 2,622 4 View

In Ansys APDL the stiffened plate girder is not getting meshed after gluing! What to do?

This is a model of a stiffened plate girder. The APDL code is provided below for its geometry generation. It is not getting meshed after gluing all the volumes. If the volume is subdivided in the...

18 January 2021 4,839 4 View

In psychometrics, what are some quant. methods to examine several competing operationalizations of the same construct to see which is most 'valid'?

13 January 2021 2,740 3 View

Can Gan be used for one class classification?

Hi everyone Is it possible to use the discriminator of a GAN network for one class classification to distinguish between positive and negative data?

14 December 2020 8,903 3 View

Why my Nyquist plot does not intersect the x-axis?

I have been measuring the conductivity of the biopolymer membrane by using the 2-electrodes connection and sometimes the plot intersects the a-axis and other times doesn't. The membrane was...

03 December 2020 8,471 1 View

What is the best method of discriminant data analysis?

I have three dataset of quantitative and qualitative liquid chromatography, GC-MS and DNA barcoding of few samples. What would be the best way to discriminate and visualize the data?

25 October 2020 1,173 9 View

What featues will give the best performance in discriminating between signal and noise?

15 October 2020 5,957 10 View

Can we use PEG 400/600 in Dissolution medium, at what level of % ?

Surfactant aid in release of poor soluble drugs but in some cases discrimination is compromised. Can we use PEG if it gives better results in terms of release and discrimination power of...

21 September 2020 6,907 4 View

What are the SPSS commands to calculate item discrimination index and difficulty index as part of item analysis?

Hi everyone! I am trying to find the "how to" on item difficulty and discrimination on SPSS and its interpretation. I have read about and performed the command analyze> scale> reliability...

21 September 2020 4,040 3 View