How could the concentration from RNA extraction is really low?

Hello everyone, currently, I'm trying to do RNA extraction from seeds of Arabidopsis. I'm using the protocol below: RNA isolation from siliques, dry seeds, and other tissues of... I use about...

17 February 2021 7,583 4 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View

SDS-Gel stacking gel polymerization issues?

Can anyone provide any insight as to why my Tris/Gly SDS-Gels are polymerizing slowly? This especially applies to my stacking gel (image attached) both 4% and 6%. The stacking gel wells polymerize...

24 January 2021 3,791 4 View

How to use back-trajectory analysis (HYSPLIT) to assess air parcels at breathing/monitor height?

Hi there, I'm currently monitoring air pollution in Grenada, West Indies and as part of my project I have identified four scenarios in which I would like to use a back-trajectory analysis...

27 December 2020 730 4 View

Origin of the term "transposition" used in photovoltaic performance modeling?

In PV modeling, the process of elevating GHI data to a tilted surface is often referred to as "transposition". However, strictly speaking, "transposition" in mathematics refers to rearranging a...

22 September 2020 767 3 View

Help figuring out why only lifted cells express eGFP after transduction?

I'm attempting to transduce HEK293T cells with a lentivirus expressing eGFP. I have been observing a low transduction efficiency and after a 24 h transduction the only cells expressing eGFP are...

03 September 2020 9,491 5 View

How to stimulate PBMC-derived monocytes with TLR7 and TLR8 agonists?

I'm trying to stimulate human monocytes from fresh PBMC isolations with 2ug/mL imiquimod and 2ug/mL ssRNA40. I do this in 10% FCS complete RPMI for 18-20 hours before staining and analysing CD14+...

10 August 2020 7,095 1 View

What are the independent, intensive parameters for multicomponent mixtures (and how do I determine them)?

Hi all, For different problems in gasdynamics/thermofluids, different thermodynamic inputs are most convenient for expressing and solving the problem. For example, if I have a stagnation process,...

23 June 2020 1,085 2 View

What literature should I read to start understanding flow batteries?

I need recomendations on the most core and fundamental materials/literature to start understanting flow batteries from scratch so I can migrate to membraneless flow batteries. Hence, I will also...

09 June 2020 6,270 3 View

Any recommendations for a high-quality, contract basis, academic illustrator for a paper in Nature Climate Change?

We looking for a high-quality, contract basis, academic illustrator for a paper in Nature Climate Change, any recommendations? Proven track record important.

26 April 2020 7,427 8 View

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02 March 2021 3,060 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View