Can E.coli BL21-Codon Plus be expressed in pDEST17 vector?

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Abhishek Sen

Dear Tsumbedzo,

pDEST17 is a bacterial expression vector. It can be used for expression in the BL21 codon plus. I suppose you are planning on heterologous expression, since only then does the BL21 codon plus prove to be handy. You could also optimize the codon you wish to insert.

Tsumbedzo tertius Tshivhidzo

BL21 codon plus is handy since i have used it in pet-SUMO plasmid vector and the expression was accurate.

and how do i optimize the condon i want to inser?t

That's great Tsumbedzo!

By optimizing I meant matching the GC %, codon usage and checking for restriction sites within your gene of interest.

i think the problem is on my plasmid not the cells