Hello, I am trying to figure out how to induce and silence the same set of neurons in brain using dreadds. I am running into the problem that CNO activates both Gq and Gi dreadds. If I infused the Gi and Gq in the same animals, then both get activated at the same time after CNO injection. I am trying to find out the population of behavior relevant (like in open field test) inhibitory and excitatory neurons involved in regulating the behavior in the same animals by using in-vivo electrophysiology. Any help will be highly appreciated.
I think that`s quite difficult. As I know by now, Gq and Gi can not be activated by the same concentration of CNO. In general, Gq need less CNO than Gi to function on cells. It might cause seizure by higher CNO.
alternatively no but if you wish to target a particular circuitry and switch off one arm/ activate the other than yes. As Minghui mentioned, higher doses of CNO are tricky and may have off-target effects (despite being said it doesnt). Better method would be looking into combining opto and inhib DREADD or ivermectin-activated Cl channels and Gq DREADD
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