Hello dear everyone's
Can combination GA and PEG be used simultaneously to study drought stress in the seeds with physiological dormancy?
Thank you in advance for your attention
Hamidreza
It is a rather vague question.
What is your research question?
What species are you using?
PEG is used to simulate osmotic stress, while GA would induce viable seed to break dormancy.
The latter is particularly useful, because allows to distinguish which seeds were unable to break dormancy themselves, but were still viable.
Thus, it is necessary to add GA after you can make that distinction.
In literature you can find species- and conditions-depending number of days, after which one can say that the seed able to break dormancy already did and the others will not.
A basic experiment would be to sow the seed in optimal conditions and under osmotic stress (with PEG), counting the germinated ones every 12h and after x days add GA (GA4+7 around 10 uM final conc.) and continue counting till the number does not increase (shouldn't take long).
Remember that GA is not soluble in polar solvents.
At different points you can analyze the transcriptome, the fresh and dry weight and other parameters of the seeds.
You could also produce transgenic lines with reporters for genes, that you hypothesize being involved in the germination
I hope to have helped
Yes, they can. But I would not call it physiological 'dormancy'. It's just how all seeds normally behave until they encounter conditions beneficial for germination :)
Andrew Paul McKenzie Pegman Dormancy is the temporary failure of a seed to complete germination under favorable conditions.
The term “physiological dormancy” is used to denote a reversible type of dormancy caused by suspending germination-related cellular activities in the embryo and it's crucial for seasonal dormancy cycling of seeds in the soil and secondary dormancy.
Simply put: ABA is the main suppressor and GA is its antagonist, therefore adding GA to growth medium will promote the germination of all viable seeds (not fruits).
Water is not all is needed for the germination of seeds.
First, the seed must be developed and still viable.
Secondly, dormancy must be broken in specific ways.
For instance Annona crassiflora require several months at
Nicolò M. Villa
Andrew Paul McKenzie Pegman
Both of you are right, of course, in your field of expertise
Thanks for your comments
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