Can cold shock cause polyspermy in fishes /sturgeon?

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How to find value for Ki (inhibition constant); Kd (dissociation constant), and Km "equilibrium constant?

Hello, I got reviver comment " please look into the comparative assessments regarding Ki (inhibition constant); Kd (dissociation constant), and Km "equilibrium constant" of the target...

09 June 2024 10,069 3 View

What can be the alternate buffer for TAPS (PH 8.5) in Post tagmentation Wash step in Cut & Tag experiment ?

I am performing Cut & Tag experiment. I was wondering if there is any alternative/replacement for TAPS pH 8.5 in single tube Tagmentation and pA-Tn5 release ( DNA purification) step of Cut and...

02 April 2024 4,214 0 View

How much of primary antibody do you add on slides in immunohistochemistry(IHC) / Immunocytochemistry (ICC)?

Most of the Antibody company (having pr. Ab conc. range 0.2 mg/ml - 0.5mg/ml) websites suggests dilution around 1:200, but it seems not staining or faintly staining. What is the hand on experience...

28 March 2024 259 3 View

Why do I get minor electric shock in my laboratory when touch something metallic?

Sometimes when I touch my colleague or metal bodies. i fell shock with some minor electric sound.

27 February 2024 1,191 3 View

How to separate dead cells from live cells when propagating suspension cells?

I am growing small cell lung cancer suspension cells. How do I separate dead cells from live cells while splitting the cells after confluency in flask?

28 January 2024 8,818 6 View

How to do multiple data sequential Rietveld refinement in TOPAS?

I can do Rietveld refinement of single multiple phase data, but I am not able to do multiple data sequential Rietveld refinement. I am using Topas V7.

21 January 2024 2,083 0 View

Why does the transmittance % in FTIR become more than 100% (Opposite direction)?

I did FTIR of a reactive dye's powder. But I see that the transmittance of 3 peaks went opposite direction of normal peaks and eventually the transmittance percentage became more than 100%. What...

17 December 2023 7,093 2 View

Does a thinner intrinsic layer in a 'p-i-n' solar cell, compared to the diffusion length, lead to increased or decreased charge carrier recombination?

As we know, when the thickness of any layer in a p-n junction solar cell exceeds the carrier diffusion length, the photo-generated carriers recombine before reaching the charge transport layer....

15 December 2023 4,930 3 View

How to calculate polymorphic information content (PIC) value for SCoT marker data? Which software helps to calculate it?

I am trying to evaluate the genetic homogeneity of wild grown mother plant and tissue culture raised plants of Piper sp. using SCoT and other dominant markers.

06 December 2023 1,837 0 View

Does enthalpy of chemical sorption reaction is a strong function of pressure and/or temperature for soprtion of ammonia in halide salt?

In most of the literature the enthaply of formation/reaction is chosen to be an constant. Some researchers claimed that in a given pressure range (say: 10 bar) this parameter can be chosen to be a...

07 November 2023 8,839 0 View

The reason for breeding Loxp mice with FLPe mice?

I am planning to use CD4 Cre mice crossed with Loxp mice. However, the laboratory from which I am trying to get the loxp sperm says they have "the product of the EMMA ko crossed with the FLPe to...

16 July 2024 7,475 1 View

Does egg white interfere with any histology staining?

I have mouse lung tissue which is mounted with egg whites.I need to stain my tissues with PAS and HE. Can egg whites interfere with these stainings?

02 July 2024 8,261 0 View

Why do men so often treat their female partners with so little respect?

So what turns her on? … A man who makes her feel ‘safe’ and treats her like she is a vital part of his very existence, and not just a favourite sperm dumpsite. (Yangki Akiteng)

22 June 2024 4,988 2 View

What can cause that mosquitoes from Rockefeller reference strain (Aedes aegypti) spend too much time in larval stages?

We have been rearing Rockefeller mosquitoes for many years in our laboratory without any problems, but lately, in spite of good viability of eggs and unchanged environmental conditions (27°C,...

20 June 2024 3,515 2 View

Effect overheating egg on nutritional value and man's health ?

Effect of heating and nutritional value loss

17 June 2024 2,159 1 View

Does water pressure dominate fish body shape?

Actually we found various fishes with typical body shape too.

11 June 2024 8,519 1 View

What is the hemagglutination assay for fiber protein of egg drop syndrome virus(EDSV) ?

How can I use EDSV fiber protein antigen for hemagglutination test?

05 June 2024 1,459 0 View

Fish eggs ID?

Any idea of the species? Catch in may, near roscoff in brittany, No oil globule, size 1.2mm The specimen is well marked with melanophore, and pectoral fins are already pigmented (as seen on...

26 May 2024 7,624 0 View

How do the Maxent use to prediction the distribution of the cave fishes in Karst area?

As the known, there were the most richness species diversity of the cave animals, especially for the cave fishes, because the underground river sytems. But there were the diffculties exist also,...

14 May 2024 9,131 0 View

Does anyone have a photo of the eggs of Harpagifer bispinis?

It's not a question, but if anyone has a photo of the eggs of the fish called Harpagifer bispinis, please share it with me.

28 April 2024 5,052 2 View