Will my revised and resubmitted paper be regarded as a new submission?

The editor commented that the manuscript in the current format is unsuitable for acceptance. However, the editor recommend to revise the manuscript in accordance with the reviewers comment and...

30 July 2020 6,272 4 View

Is there any material which is rigid in normal state and liquifies when vibrated?

Is there any material which changes into liquid state when given certain vibration such as earthquake? As we know that when we vibrate saturated sand, liquefaction takes places and the sandy soil...

27 January 2020 2,464 3 View

How to specify 'tangential velocity component' as an input parameter in ANSYS fluent?

I'm using a mass flow inlet boundary condition with cylindrical coordinates. I want to parameterize the tangential velocity component. However, doing so by defining an input parameter...

25 August 2019 7,331 3 View

What may be the possible reasons for getting only a single band during plasmid isolation?

Plasmids are always present in multiple forms. What might be the issue with my strain?

11 January 2019 5,119 1 View

Screening of a deletion mutant produced by triparental conjugation in a bacteria

27 October 2016 4,661 3 View

Does anyone know about allele specific PCR?

I did gradient of allele specific PCR but haven't got amplification. So, please tell possible check points?

20 November 2015 2,688 4 View

Temperature sensitivity of plant growth hormones

What is the temperature sensitivity of plant growth hormones?

07 August 2012 7,080 6 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View