Fallowing link may be helpful to u:
http://onlinelibrary.wiley.com/doi/10.1111/mec.12475/abstract
Thank you dear Santosh. This paper works out DNA based analysis ... Is it necessary of metagenomic approach of analysing snakes´diet ?
Analyses of stomach contents and feces (scat) are proven low-tech but time-consuming methods. Stomach contents and a bit easier because prey items are often still readily recognizable. Scat analysis, on the other hand, requires a lot more effort since you are usually working with only keratinous structures like hair from mammals, feathers from birds, scales from squamate reptiles, and exoskeleton pieces from arthropods (the latter not keratin but chitin, I believe, but undigestable nonetheless). In my experience with hair and lizard scales, I found identification scales to be rather straight forward when you have preserved specimens of the candidate species at hand for comparison. Mammal hairs are another matter, requiring the assembly of a reference set of the guard hairs mounted on microscope slides of all candidate species, followed by careful dissection of the feces and the sorting, cleaning, and mounting of representative hairs on slides and lots of microscope work. If you're really lucky, some mammal remains will contain teeth or other bone fragments that can be compared to museum specimens with much more ease. Good luck!
Dear Prof. Michael, many many thanks for the clear idea of working with stomach or faecal samples. Finding reference materials looks tedious job in context of Nepal.
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