Hi, I have not done this myself, but I would compare leaves in light and darkness, or use a treatment with abscisic acid to force the closure of stomata. In any way you have to assess the degree of stomatal closure. You could measure stomatal conductance or do imprints. This is not a solution, but maybe it gives you some ideas.

If you got access to IRGA (Infrared Gas Analyzer) then you can take stomatal closure/opening and rate of transpiration and photosynthesis readings through this instrument. This is a common physiological analysis equipment easily accessible (on request) from any crop physiology department or R&D institute in your country.

I agreed with imran . Instead of IRGA you can also use porometer that measures stomatal conductance using a sensor head with a fixed diffusion path to the leaf. Porometer determines stomatal conductance by measuring the actual vapor flux from the leaf through the stomates and out to the environment.

Thanks Imran and Tiwari. I know that But i want to estimate the cuticular transpiration separately.

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this article may help you

http://www.ncbi.nlm.nih.gov/pubmed/11520878

Thank you Tiwari. I have already collect it

https://www.researchgate.net/publication/230794770_Stomatal_and_Cuticular_Transpiration_of_Greenhouse_Tomato_Plants_in_Response_to_High_Solution_Electrical_Conductivity_and_Low_Soil_Water_Content

Article Stomatal and Cuticular Transpiration of Greenhouse Tomato Pl...