I want study the antibacterial and antifungal studies for my synthesized compounds. So i want to know which standard good for this? (In vitro)
I suggest starting with test method ASTM G-21, Standard Practice for Determining Resistance of Synthetic Polymeric Materials to Fungi
http://www.jclmm.com/qk/201412/8.pdf
you can follow methods given in the article
Dear Dhandapani Arumugam,
The following is a recent review article depicts all commonly used methods to screen antimicrobial activities. Among these methods: disk-diffusion, well diffusion and broth or agar dilution and etc. In our lab we use disk-diffusion which is considered to be efficient and easy to handle.
Journal of Pharmaceutical Analysis
Volume 6, Issue 2, April 2016, Pages 71–79
Open Access
Review Paper
Methods for in vitro evaluating antimicrobial activity: A review ☆
Mounyr Balouiri, ,
Moulay Sadiki,
Saad Koraichi Ibnsouda
doi:10.1016/j.jpha.2015.11.005
Get rights and content
Open Access funded by Xi'an Jiaotong University
Under a Creative Commons license
Abstract
In recent years, there has been a growing interest in researching and developing new antimicrobial agents from various sources to combat microbial resistance. Therefore, a greater attention has been paid to antimicrobial activity screening and evaluating methods. Several bioassays such as disk-diffusion, well diffusion and broth or agar dilution are well known and commonly used, but others such as flow cytofluorometric and bioluminescent methods are not widely used because they require specified equipment and further evaluation for reproducibility and standardization, even if they can provide rapid results of the antimicrobial agent's effects and a better understanding of their impact on the viability and cell damage inflicted to the tested microorganism. In this review article, an exhaustive list of in vitro antimicrobial susceptibility testing methods and detailed information on their advantages and limitations are reported.
http://www.sciencedirect.com/science/article/pii/S2095177915300150
In addition, the following links contain protocols for antimicrobial screening:
http://www.microbelibrary.org/component/resource/laboratory-test/3189-kirby-bauer-disk-diffusion-susceptibility-test-protocol
http://amrls.cvm.msu.edu/microbiology/detecting-antimicrobial-resistance/test-methods/examples-of-antibiotic-sensitivity-tesing-methods
Hoping this will be helpful,
Rafik
Hello Dhandapani. For antibacterial investigation, you can easily do disc diffusion test and/or well diffusion test depending whether your compound can be impregnated on filter papers. Then you can find the minimum inhibitory concentration (MIC) of your compound against the bacteria using the scheme for preparing dilutions of your compound by CLSI (2016) (link here: http://em100.edaptivedocs.net/dashboard.aspx). You can also do 24hr/48hr kinetic growth studies and time-kill assays to observe hourly effects of your compound on the bacterial growth. All the best!
Thanks for everyone response to my questions?
I suggested to do disc diffusion method for microbial screening. I need reference standards for the bacterial and fungal. For ex: ampicillin, miconazole, etc.,
So i need recent and easily available reference standards.
In our laboratory we have tried strptomycin for bacteria and nystatin for fungi. If you are testing Candida albicans as one of the pathogen then better try both standards. Though Candida albicans is a fungus some times you may not get inhibition zone for nystatin as it grows both as a yeast of filamentous cells. In that case streptomycin for Candida albicans
- Badges
- Science topic
- Similar topics
- Chemistry
- Organic Chemistry