I want to suppress the Basic Leucine Zipper ATF-Like Transcription Factor 2 protein in mice model. Is there any chemical or protein which can inhibit this protein production?
I want to clone ZAP about 2100bp. I am facing difficulties to clone this gene can anyone recommend me a best method and vector to clone. I want to use this clone for A549 cell line
05 June 2018 3,939 2 View
Is there any inhibitor available to suppress the ZC3HAV1 (ZAPs) protein in live mice? Or recommend any quick method to knockdown the gene in mouse model
04 May 2018 1,147 0 View
I am trying to produce stable A549 cell lines targeting my gene knockdown by shRAN. When cells get transfected in next passage they start to die very fast while the cells which are not cloned they...
05 June 2017 4,812 5 View
need to review articles on care giving in dementia and challenges faced
12 August 2024 6,424 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...
06 August 2024 6,227 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View