About loading of Doxorobicin on MOF (drug delivery)?

Dear Researcher Respectfully, I am working on drug delivery. I synthesized Zn-MOF. I am going to load Doxorobicin on it and the study the derivery behaviour. I tried several times to load. but I...

07 February 2021 748 2 View

How can I represent a 2-parameters function in series type?

I want to represent a function like f(x,y)=sin(1/(x+y)) about [x,y]=[0,0] in series type. how can i do it in matlab or maple?

10 January 2021 8,389 5 View

Can you recommend a protocol for immunohistochemistry of mouse brain sections using anti-MAP2?

Current protocol does not give clear images.

04 January 2021 1,614 3 View

Challenges in growing deep learning model?

I implemented an image classifier and object detection model. I added a new class every day to my model and the data set growing too. I wanted to ask if anyone has the same experience? at now it...

03 January 2021 4,652 5 View

Anyone knows how to extract RNA from samples with high starch content (wheat) by using RNeasy Plant Minikit?

I am using 74904 to extract RNA from wheat seed and I have tried both of the lysis buffer. As it is mentioned it protocol, buffer RLT is the lysis buffer of choice but it may cause...

14 October 2020 9,409 7 View

What causes different microstructure in a sample?

I have synthesized high entropy oxide with (Ni Zn Cu Mn)1-x Cox composition. At x=0.05 the microstructure became rod shape and the SEM picture of the other part of that sample is different. And...

12 October 2020 5,576 5 View

How to increase zebrafish survival rate after spinal cord injury ?

Hi everyone I'm having trouble with the high mortality rate of my zebrafish after surgery. ( the surgery is done based on this article: Becker, C. G., Lieberoth, B. C., Morellini, F., Feldner,...

13 September 2020 6,781 1 View

Antibiotic screening of drug resistance cancer cell line with MDR pumps?

Hi. during our study, we faced with a problem in the screening of EPG RDB 257 cell line which is a resistant cell line due to the expression of efflux pumps such as MDR 1 which actively excrete...

27 August 2020 9,192 1 View

How to work with IQF device with the highest efficiency?

Hello I work with the IQF machine My problem is that when wet vegetables enter the machine, the efficiency of the machine decreases. What can be done to remove moisture from vegetables without...

01 August 2020 8,179 1 View

In response surface methodology, can we use sum of the response signals for two dependent variables as a function of independent variables?

RSM based on CCD is supposed to be applied for finding the optimum value of some independent variables. There are two dependent variables. Can we use sum of the response signals obtained for these...

04 July 2020 4,865 7 View

What is the cause of the banding obtained after purifying the PCR product with ExoSAPIT?

Hello We are working on a PCR product with an approximate size between 5000 and 7000 bp, we want to use it as a positive internal control, since we have sequenced it and it corresponds to the...

17 February 2021 2,896 3 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

Antibody fragments after Dha (Dehydroascorbic acid) re-oxidation?

Hi, I am trying to re-oxidize the disulfide bridges of an antibody with DHA. I always have some good amount of HL fragments (heavy + light chain fragment), despite varying reaction times, pH,...

31 January 2021 9,495 2 View

Gibson PCR and reaction challenges, any tips?

Hi I am having issue's with Gibson assembly, seeing as I am new in the filed of cloning, expert feedback would be very much appreciated I am trying to clone two fragments (frag 1 [185bp, 145bp w/o...

29 January 2021 1,406 2 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

I am having trouble with cloning my gene. How can I fix this?

16 January 2021 6,566 8 View

Why am I getting 2 bands for Gibson Cloning Vector PCR?

Hi, I designed Gibson primers (2 primers for fragment and 2 primers for vector) using snapgene. Then I ran PCR separately for Fragment and Vector. Fragment DNA was found to be the correct sized...

11 January 2021 9,500 2 View

Does Tea Garden proves to be an alternative to forest landscape?

Is there possible that the Tea Gardens proves to be an alternative habitat to forest dwelling organisms? If yes, what pressure do they face in relation to anthropogenic pressure?

03 January 2021 429 13 View

Why do I keep obtaining the same secondary PCR band along with the specific product band?

Hello everyone! I need to amplify a specific DNA fragment in order to do Sanger sequencing for the detection of a point mutation in human patients. The fragment is in an exon of my gene of...

25 December 2020 1,805 3 View

Has anyone measured split YFP in a Tecan (Bimolecular Fluorescence Complementation)?

Hello, I've been trying to measure split YFP-constructs using a Tecan. The problem is, i can detect YFP as a whole construct without any problems (so the excitation/emission wavelengths are fine)...

15 December 2020 2,209 1 View