I want to do western blot of micromanage protein fraction. Its better if i can use the cell wall protein of my microalgae. I shall be appreciate if some one help me giving some protocol.
In principle, you can use methods established for preparation of chloroplast membrane proteins. But, my first question would relate to purity of the algae membrane fraction you intend to use: How to you check absence of contamination by the fraction of "soluble" proteins? Are you using sort of leaching protocol, i.e. are you monitoring protein patterns during purification?