I am screening mulberry germplasm for salinity tolerance in pots which can hold 17 kg soil. I am observing wide variability among the mulberry accessions at EC 8 dS/m. Variability in necrosis, root characters, and leaf Na+ content and Na+/k were observed.
We raise the samplings from stem cuttings and plant them in pots after 4 months and impose the stress using Nacl, MgSo4 and CaCl2. We maintain these plants for 70 days and record the observation. But, since it is pot culture, I was asked to field validate the results. Can anyone suggest some simple molecular work to prove the resistance?
Dear Lakshmi ;
I don't have much experience in this, but your question is much more interesting & I also had this type of question regarding Rice. From my experience, I'd like to say ;
1. first you better screen your germplasm for salinity tolerance.
2. find out most diverse germplasm (cultivar / variety) for two extremes ( Highly salt tolerance & highly salt susceptible) by phenotypic parameters
3. then by using appropriate crossing method you try to cross " Highly susceptible cultivar X Highly resistance cultivar
4. Now you can screen Parents and F1 generations by related markers ( If you use more # of markers, you can lead to a better performance)
5. Finally you can compare markers and plant phenotypic characters and would be able to come to a conclusion where $$$ marker (s) are linked with salt resistant / tolerance
This is the very simple method that comes to my mind, but you need more time and must have a good knowledge of the crop ( mulberry) botanical / physiological parameters / life cycle / agronomic practices... etc.
Don't know whether this would help you??
Good Luck.
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