I need to do an experiment on enzyme kinetics by using Stopped Flow instrument. But due to lack of instrumentation facility I am unable to progress in my project. Can anyone suggest me where I can avail the facility in India?
I purified two ~ 55KDa proteins having C-terminal His-Tag. The concentration of both the proteins are ~ 40 uM. One is the wild type and the another is the mutant protein. The proteins always...
02 March 2018 6,566 7 View
I need to do an experiment "Tandem Affinity Purification". I need a vector with TAP tag
01 February 2018 2,411 0 View
I am trying to clone an E.coli gene of 2106 bp into an expression vector. The first problem faced while cloning is that the PCR is not getting optimised. 3 Non-specific bands are getting...
01 February 2016 5,661 6 View
I cloned a gene in pET28a(+) vector and transformed in E. coli BL21 for expression. After IPTG induction and SDS PAGE analysis I am getting 3 bands that seem to be induced together instead of one...
05 June 2015 5,740 9 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
or we can collaborate
01 August 2024 6,705 2 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...
25 July 2024 2,309 5 View
Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...
25 July 2024 9,058 4 View
I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...
24 July 2024 6,592 5 View