Sagarika Das

5 Questions 8 Answers 0 Followers

Questions related from Sagarika Das

Do very low concentration of non-specific bands in purified protein sample interefere in Circular dichroism results ?

I purified two ~ 55KDa proteins having C-terminal His-Tag. The concentration of both the proteins are ~ 40 uM. One is the wild type and the another is the mutant protein. The proteins always...

03 March 2018 6,548 7 View

Can anyone in India send me a vector with TAP tag ?

I need to do an experiment "Tandem Affinity Purification". I need a vector with TAP tag

02 February 2018 2,398 0 View

Can anyone suggest me where I can avail the Stopped Flow instrumentation facility in India?

I need to do an experiment on enzyme kinetics by using Stopped Flow instrument. But due to lack of instrumentation facility I am unable to progress in my project. Can anyone suggest me where I can...

12 December 2017 8,327 0 View

Why the PCR product after restriction digestion is showing two bands?

I am trying to clone an E.coli gene of 2106 bp into an expression vector. The first problem faced while cloning is that the PCR is not getting optimised. 3 Non-specific bands are getting...

02 February 2016 5,635 6 View

I am facing a problem in Protein Expression. Why am I getting 3 induced bands instead of one?

I cloned a gene in pET28a(+) vector and transformed in E. coli BL21 for expression. After IPTG induction and SDS PAGE analysis I am getting 3 bands that seem to be induced together instead of one...

06 June 2015 5,720 9 View