02 February 2018 0 2K Report

I need to do an experiment "Tandem Affinity Purification". I need a vector with TAP tag

More Sagarika Das's questions See All
Do very low concentration of non-specific bands in purified protein sample interefere in Circular dichroism results ?

I purified two ~ 55KDa proteins having C-terminal His-Tag. The concentration of both the proteins are ~ 40 uM. One is the wild type and the another is the mutant protein. The proteins always...

02 March 2018 6,566 7 View

Can anyone suggest me where I can avail the Stopped Flow instrumentation facility in India?

I need to do an experiment on enzyme kinetics by using Stopped Flow instrument. But due to lack of instrumentation facility I am unable to progress in my project. Can anyone suggest me where I can...

11 December 2017 8,348 0 View

Why the PCR product after restriction digestion is showing two bands?

I am trying to clone an E.coli gene of 2106 bp into an expression vector.  The first problem faced while cloning is that the PCR is not getting optimised. 3 Non-specific bands are getting...

01 February 2016 5,661 6 View

I am facing a problem in Protein Expression. Why am I getting 3 induced bands instead of one?

I cloned a gene in pET28a(+) vector and transformed in E. coli BL21 for expression. After IPTG induction and SDS PAGE analysis I am getting 3 bands that seem to be induced together instead of one...

05 June 2015 5,740 9 View

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How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

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Transfection in HEK293T cells?

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What is the relationship between protein structure and N or C terminal tagging choosing?

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Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

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Dear fellow researchers studying in molecular biology, are there any tips on how to avoid plasmid dimerization when extracting plasmids?

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