Can anyone suggest me to get antibacterial and antifungal effect with different way without plate method.
you can use turbidometric method of assay where you can measure turbidity at 530nm and compare it with a standard.
Do you mean the microtiter plate method? Try disc difussion method
very simple method by TLC , spray the TLC with microorganism and incubate it , then spay wth the plant extract leave it and see the results
i think in this case either u purify ur sample and try on HPLC , or buying standardsvfor ur work
For anti-fungal effect you can use that
See above
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