hi,
I have to do enzymatic assay with active protein located in the nucleus, for that i need to isolate nuclear protein / extract from Arabidopsis leaves.please send me the proper protocol for the nuclear extraction.
Thank you for asking such a nice question. Below mentioned two links may help you.
Nuclear Extraction from Arabidopsis thaliana
https://bio-protocol.org/e306
Arabidopsis thaliana nuclei purification protocol
https://www.germainhugo.com/protocols/a-thaliana-nuclei-purification/
Thank you
Yes, the nuclear extraction method available in bio protocol is a good one as suggested by Mohammod Hussain.
https://bio-protocol.org/e306
We usually use this method
Liechtenstein C, Draper J (1985) Genetic engineering of plants. In:
Glover DM (ed) DNA cloning, vol 2. IRL Press, Oxford,
pp 67–120
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