Shobhita Saxena

3 Questions 3 Answers 0 Followers

Questions related from Shobhita Saxena

Can anyone suggest me the protocol for nuclear protein isolation from Arabidopsis leaves?

hi, I have to do enzymatic assay with active protein located in the nucleus, for that i need to isolate nuclear protein / extract from Arabidopsis leaves.please send me the proper protocol for the...

05 May 2019 8,248 3 View

Can anyone one suugest me the full protocol for immunoaffinity protein purification fron Arabidopsis?

I have to check the methylation activity of my protein so i referred your paper JMJ14 is an H3K4 demethylase regulating flowering time in arabidopsis (Cell Research). so can anyone send me the...

01 January 2019 2,819 4 View

Problem with cloning PCR, can't amply the full-length cDNA with PCR?

i am trying to clone Arabidopsis gene from cDNA. first time i got amplification with both taq and phusion poll.but after that i am getting any amplification from same cDNA sample. i have tried all...

07 July 2018 226 7 View