Can anyone suggest me the protocol for AROMATASE ENZYME assay from fish brain and gonad?

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Which column should I use for metabolite identification in a human plasma sample?

Please suggest the appropriate column and its specifications for metabolite identification in human plasma samples. Your recommendation will be greatly appreciated.

28 May 2024 7,789 0 View

Multilevel modelling- number of clusters required (how much) for the level two predictors?

Dear all, I am applying a multilevel modelling into my dataset. Level 1 is induvial patients data and level 2 is community. Outcome variables is "Health service utilisation (Yes/No)" and level 2...

09 August 2023 9,092 3 View

Protein hits low with Hela QC for QExactive HFX?

Hi, I have a QE HFX and last winter the protein hits dropped and the field engineer found all evaluations on the MS passing but hits did not improve until the electronics board was replaced. RF...

26 March 2023 1,256 0 View

How to extract saccadic amplitude for each trials from eyedata in .mat format ?

I have a matlab structure eye data from SRR eyelink. How to combine behavioral data with the eye data and extrcat saccadic amplitude for each trial.

31 January 2022 3,270 1 View

Is "INTERNATIONAL JOURNAL OF COMPUTER SCIENCE AND NETWORK SECURITY" (eISSN 1738-7906) a scholarly and good Journal ?

INTERNATIONAL JOURNAL OF COMPUTER SCIENCE AND NETWORK SECURITY eISSN 1738-7906 is indexed in web of science. But this journal is listed in the predatory list and Beall's list. This particular...

20 May 2021 3,784 2 View

How to compute of capacitance from a 3D potential distribution between parallel plate capacitor?

I have evaluated the 3D potential distributions between parallel plates of a disc capacitor .I used boundary condition as 1V-0V for solving laplace equation. I calculated the capacitance from...

10 April 2021 8,133 4 View

Which are the quality parameters considered for injection molded plastic products? And which inspection tools are used for the same ?

Quality parameters and inspection tools may differ from product to product. Please write whichever you know.

10 December 2020 1,685 2 View

How long after autoclaving pipette tips does sterility last?

I'm currently autoclaving boxes of pipette tips weekly. I use them every day, so the boxes are constantly being opened and closed. How long can I reasonably expect them to stay sterile?

10 September 2020 6,950 1 View

Can you suggest any [powder defoamer which can be prepared in lab for PVA ?

I am looking for a composition and preparation of powder Anti-foaming agent for Adhesives and thickeners like Polyvinyl alcohol. It would be very helpful if i receive answers. Thank you.

07 July 2020 3,958 1 View

Autophosphorylation of IKKa and IKKb or non productive hydrolysis?

I am developing a biochemical assay for IKK-alpha and IKK beta. I got the proteins (active kinases) from Signal Chem. What I see is ADP production with ATP+IKKtide (my acceptor substrate) and...

20 May 2020 9,910 0 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Can telekinesis, telepathy, and prediction be achieved through science?

Information in The Three Dimensions of Time.Information in The Three Dimensions of Time Information Transfer based on Brains Entanglement.Information Transfer based on Brains Entanglement Time...

28 July 2024 7,522 0 View

Why only alpha wave of brain are found in some patients?

I have found an EEG where only alpha waves are present. Beta waves are not found in active patients. What interpretations ?

26 July 2024 4,741 1 View

How to add viscosity into a hyperelastic material such as Mooney-Rivlin rubber in LS-DYNA?

Hi, I am working on a head FE model and want to try different material types on brain. I read some papers that use Mooney-Rivlin rubber model and wondering if i can add viscosity into it. Is it...

24 July 2024 5,908 0 View