I want to do seed viability test using tetrazolium chloride (TTC) method. Any suggestion from the experts will help me in this matter.
Thank you.
See:
Ooi M, Auld T, Whelan R 2005. Comparison of the cut and tetrazolium tests for assessing seed viability: a study using Australian native Leucopogon species. Ecological Management and Restoration 5: 141–143.
I used the cut test, which is outlined in the above paper and was found to be reliable and easier :)
Sir,
You can use the chemical like 2,3,5 Triphenyl tetrazolium chloride. before conducting the test you have preconditioned the seed samples i.e soaking of seeds in water for particular duration and expose the embryo in the solution and incubate it for atleast 1- 2hours and observe the sample
Yo have to use 2,3,5,triphenyl tetrazolium chloride, the seeds are preconditioned, by inmersing in water or between moist papers, all night for example, in a temperatura lower than the temp of germination. After that, according the seeds caracteristics, you should cut the seeds or eliminate the teguments, etc, to allow de tetrazolium penetrate the seed. When the seeds are thus prepared, you should inmerse then in te solucion of tetrazolium (0,1 to 1%), in dark a 35ºC, for 2 to 3 hours, then wash the seeds in water and observate the dyed parts of seeds. You can consult the INternational Rules for Seed Analysis (ISTA), where you can find the protocol for each seed.
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