Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Sample modeling in ABAQUS software and its relationship with the real world?

About crack modeling in ABAQUS: Mode I (pure-tensile cracking) and Mode II (pure-shear cracking), these assumptions may be accruing in theoretical approach (ABAQUS) not practical in Lab....

19 February 2021 6,156 1 View

Making my research papers available for readers?

17 February 2021 8,533 2 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View

Alternatives to .45 um filter to isolate MS2 from lysed E. coli?

Hello, I am looking for alternatives or cheaper materials to isolate out MS2 from E. coli following lysis via chloroform. Some literature I have reviewed uses a .45 um nanopore filter following...

02 February 2021 807 3 View

Testing of Antennas?

So, I came across, where antennas are being tested in a building ( a plant), where all sorts of big equipment are around and of course the ceiling. lot of multipath which can affect the...

21 January 2021 9,437 3 View

Spectrabase no longer has overlay function?

Hello, I am a quality control chemist and I test raw materials for a nutritional supplement company. I make heavy use of FTIR-ATR for identification. We are a small laboratory with a limited...

07 January 2021 8,850 2 View

How to calculate the flow rate required for fluidization for a fluidized bed gasifier ?

We need to calculate the flow rate of air required for fluidization of the bed. We have a compressor which gives air at 7 bar and a 1 bar rotameter for measuring the flow rate in LPM. How can I...

16 December 2020 2,092 3 View

How can we simulate a pipe flow as a 2D model in fluent?

Hi I am going to model particle fluidization in a pipe using 2D simulation. To compare the Fluent result with experimental data, how can I select an appropriate width an length for 2D model by...

10 November 2020 3,610 5 View

How to calculate stoichiometric coefficient of coal devolatilization?

I am trying to model the coal gasification on fluidized bed. For this I need to define the chemical reaction of coal devolatilization in Fluent. Could you please help me how we can determine the...

21 October 2020 5,891 3 View

Does xylene and ethylbenzene contribute/affect COD and BOD reading in wastewater?

I'm currently troubleshooting a series of 3 activated carbon beds. The carbon beds are using Granular Activated Carbon(GAC). This activated carbon bed set up is to treat wastewater Chemical Oxygen...

20 October 2020 3,531 3 View

Would you please advise me how to use forecast or predict (out-of-sample)?

Hi, I just run ARDL model in which I have defined "Ln(Budget Deficit/GDP) as Dep-Var and Ln(G/GDP)+Ln(OilRevenue/GDP)+Ln(10%rich/10%poor)+.... some other political-economic indicators as...

19 October 2020 8,641 8 View