I have been trying to estimate some biochemical parameters in urinary bladder but homogenization is problem with me as indicated by very low amount of protein in homogenate.
Hi - are you using fresh or frozen tissue? I have homogenised whole bladders for RNA extraction previously and always used frozen bladders (i.e. still solid during homogenisation) with no issues in RNA but I guess protein might be different!
thank you.
but i used fresh tissues.. As said, i need it for measurement of oxidative stress otherwise for western blotting there s no problem with it.
Would snap freezing damage the proteins you are interested in? Might be worth testing out as it's much easier to break down the tissue in a frozen state. Good luck!
i m interested in measuring oxidative stress, MDA and GSH in tissue homogenates not going for blotting
- Badges
- Science topic
- Similar topics
- Cell Biology
- Methods