I would like to evaluate the effect of iron on inflammatory Cytokines...............
Please see this reference in NCBI
"The dose-dependent effects of chronic iron overload on the production of oxygen free radicals and vitamin E concentrations in the liver of a murine model."
Have you considered feeding a iron deplete or iron replete diet? It might be more relevant physiologically.
Ferrous sulfate, peroral, I can't remember the dose we used, but you will find it here http://www.ncbi.nlm.nih.gov/pubmed/22728935
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