whats the problem? just imitate natural habitat ie temperature and pH. You have pH meter, you can mesure temperature.......

Dear Tomas,

Thank you for your reply. I appreciate your answer is good. At the same time when we see the literature people are followed different type of media and pH for isolation. The same procedure i followed. Our soil sample pH is 6.02. The literature said 7.2 or 7.3 is suitable pH. When we think of use diffrent media there also different ph for diffrent media. Anyway i will try your suggestion.

thank you sharing your comments and suggestions

There might not be a general protocol for your problem - too many different species are out there. I would try different media and methods in parallel - and you will see that there are different strains you isolate. But be careful: the pH measured at room temperature is by far not the same at elevated temperature. Use proper correction and measure at the temp. used. Different buffers react different to temp. change too.

A good basis for the medium surely is imitating the source conditions as Tomas said. Add buffers but not too many nutrients and salts - some organisms do not grow in rich media, but some do.

Veeramuthu,

yes, if I were you I would try general media used for actiomycetes cultivation, but I would change pH and temperature. my knowledge in this field is rather shallow (I work with intracellular insect bacteria). As I know actinobacteria are cultivated on different media (same isolate grows differently on different media), pH is 7.3 and temperature is elevated at 27 degrees of celsius. Maybe you can try to contact people who isolated a lot of strains, like these guys I know personally: http://www.actinomycetes.cz/staff.php

GOOD LUCK