Dear Suneha;

There are different analytical methods for lipases activity determination including volumetry, spectrophotometry, immunoassays, radioactive assays, chromatography, biosensors and others.

According to your inquiry, a "spectrophotometric assay of lipase activity assay" is the matter of question. Up to my knowledge, lipase assay using p-nitrophenylpalmitate (pNPP) as a substrate may be the predominant procedure. The protocol is based on lipolysis of pNPP into yellow colored p-nitrophenol which can be colorimetrically measured at 410 nm.

 With some drawbacks, the method is still applied in many studies with intensive care about pH leveling.

You can find enclosed more details about the suggested procedure.

Also, different extraction methods of lipase from plant tissues (more specifically; seeds) are included.

Moreover, you can have a look on a formerly- answered question on the ResearchGate via the following URL;

https://www.researchgate.net/post/Could_anyone_suggest_me_a_para_nitrophenyl_palmitate_solution_for_lipase_assay

 Hope this would help.

Thank you Bardees . I will soon try your recommendations.