Dear Syed,

Please see attached file:

Apoptosis Detection

Kits

CYTOC-OX1 Cytochrome c Oxidase Assay Kit For the determination of cytochrome c oxidase activity in mitochondrial soluble and membrane

Sufficient for 100 tests bound samples. Cytochrome c oxidase [EC 1.9.3.1.] is present in mitochondria of the more

highly developed cells and in the cytoplasmic membrane of bacteria. This enzyme provides

energy for the cell by coupling electron transport through the cytochrome chain with the

process of oxidative phosphorylation. Cytochrome c oxidase is located on the inner mitochondrial

membrane that divides the mitochondrial matrix from the intermembrane space

and traditionally it has been used as a marker for this membrane. The colorimetric assay

measures the decrease in absorbance of ferrocytochrome c caused by its oxidation to

ferricytochrome c by cytochrome c oxidase.

PCR Primer Sets

APO-PCR Apoptosis PCR Bax/Bcl-2 The kit is useful for studying the progress of apoptosis and the evaluation of potential

Multiplex Primer Sets apoptosis inducers.

Sufficient for 50 (50 ml) PCR reactions Bcl-2 is a member of a large gene family encoding proteins that can either inhibit (e.g. Bcl-2,

Bcl-XL ) or promote (e.g. Bax, Bcl-XS, Bak) apoptosis. Bcl-2 inhibits apoptosis by preventing

the release of apoptosis inducing factor (AIF) and cytochrome c from the mitochondria. If

the Bcl-2 level is higher than the Bax level, apoptosis will be inhibited. The ratio of Bcl-2

to Bax in the cell can determine whether or not the cell initiates apoptosis or survives.

Some cancer cells such as melanoma overexpress Bcl-2 preventing apoptosis and allowing

malignant growth to continue.

The kit contains primer sets for the detection of Bax and Bcl reverse transcribed from mRNA

from rat, mouse or human tissues or cell lines (reverse transcriptase and thermostable

DNA polymerase not included). A primer set for a “housekeeping” gene, GAPDH, as an

internal control is included in the kit. These primer sets are optimized for the simultaneous

amplification, detection and comparison of Bax and Bcl-2 mRNA levels. The sizes of the

amplified products are 487bp for Bax, 127bp for Bcl-2 and 349bp for GAPDH.

Features and Benefits:

• Simultaneous RT-PCR amplification and detection of Bax and Bcl-2 mRNA levels.

• Includes primers for the GAPDH gene as an internal control for comparison of Bax and

Bcl-2 mRNA levels to allow for semiquantitative evaluation of Bax and Bcl-2.

• Functions with Taq DNA polymerase, AccuTaq™ LA DNA polymerase, REDTaq™ DNA

polymerase, and equivalent DNA polymerases.

• Primer solutions contain both sense and anti-sense primers of the specific gene for

convenience.

• The primers work with cDNA reverse transcribed from RNA (mRNA and total RNA)

extracted from cells and tissues using various extraction methods.

• Works in one step RT-PCR procedures.

• Genomic DNA contamination in the RNA will not be amplified.

Apoptosis PCR Primer Sets Synthetic oligonucleotide primers for RT-PCR analysis of mRNA expression. Amplifies reverseSufficient

for 50 reactions transcript mRNAs from human, mouse and rat sources. Set includes an upstream and

downstream oligonucleotide.

B 3055 Bad PCR Primer Set

B 3180 Bak PCR Primer Set

B 8304 Bax PCR Primer Set

B 9179 Bcl-2 PCR Primer Set

F 8425 Fas PCR Primer Set

F 8300 Fas-Ligand PCR Primer Set

Hoping this will be helpful,

Rafik