Strategies for short hairpin RNA interference cassettes?

In your experience, how much does the distance between the U6 promoter and the annealed -short hairpin - oligonucleotides affect the RNAi cassette expression? How to clone a shRNA sequence...

16 January 2021 9,418 2 View

How can I calculate parameters of pseudo-second model for adsorption processes?

07 December 2020 3,541 9 View

How to run a statistical comparison of same group and 2 conditions in fMRI?

Hi everyone, I have some doubts about which statistical test to apply in order to test the probability of eventA to happen at the same time of non-eventB. Basically I have my fMRI group with...

14 September 2020 1,887 3 View

What is the best way to approach building a custom polypeptide?

I am trying to build a custom poly peptide that is ~50 amino acids in length, and it will ideally have a charge of ~+4 at pH 7. I can get the positively charged attributes from incorporating a...

08 December 2019 3,597 1 View

Olsen method, problem with calibration curve. How to fix?

Dear all, we are trying for the first time to perform the quantification of P with the Olsen method. We had followed the protocol proposed by Guide to laboratory establishment for plant nutrient...

20 September 2019 1,116 4 View

Whole Blood Over Recovering Compared to Blood Plasma?

My Immunoassay is giving higher analyte concentrations for the same sample before plasma separation vs after plasma separation. For instance, I run my assay with whole blood, and I get a reading,...

10 July 2019 7,391 3 View

Is there a valid and reliable scale for measuring the quality of student-teacher relationships from the student perspective?

Is there a valid and reliable scale for measuring the quality of student-teacher relationships from the student perspective, that has been used with primary age children? Is there any research to...

04 June 2019 10,015 1 View

How to implement the GLCM (gray levels co-occurrence matrix) matrix to determine parameters such as entropy, kurtosis, and others,using python+OpenCv?

How to implement the GLCM (gray levels co-occurrence matrix) matrix to determine parameters such as entropy,kurtosis, among others of an image, using OpenCV and scikit-image libraries?

26 July 2018 5,722 1 View

Has anybody built a survey questionnaire with already validated constructs from other surveys?

HI, do you have experience with using already validated questions (for various constructs, e.g. construct to assess risk behavior, construct to assess school performance) in your own survey...

05 November 2017 6,342 3 View

Can I use mixed methods if i have only quantitative data?

28 September 2017 6,553 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View