What is the red autofluorescence in my HL60 cells?

A subset of my HL60 cells has red autofluorescence in the far red region (FL-3 > 670 nm). I first noticed this some months ago when I was checking an antibody with a PE-Cy5 tag by flow...

06 December 2020 6,005 2 View

Best ways to collect saliva from guinea pigs?

Hello, I am planning a reserch which involves analysis of saliva cortisol in guinea pigs. Unfortunately, I am currently having trouble collecting saliva samples from my guinea pigs. I am not able...

30 September 2020 9,226 1 View

Transfection in HFL-1 cells?

I am trying to transfect HFL-1 cells with a plasmid DNA. I used Lipofectamine 3000 from ThermoScientific, however the cells seem to react quite sensitive to the transfection (tried different...

17 November 2019 484 2 View

Is there a full list of scales from the FOCUS Questionnaire (van Muijen et al., 1999)?

Hi! I'm currently working on my Master's thesis and looking for the evaluative scales for the innovation and rules value orientation from the FOCUS Questionnaire developed by van Muijen et al....

05 May 2019 4,039 3 View

I have putted the 10fold amount of 2-Mercaptoethanol in my stripping buffer by mistake. Does anyone have experience with such a mistake?

I think my proteins on the PVDF membrane are gone, because there is nothing to detect anymore. But on the other hand the membranes smell after a lot of washing procedures still of 2-ME. Maybe my...

18 July 2018 171 3 View

Is there any relevance theoretical work that addresses the question of folk psychology?

This question is in connection with an article I am writing. Relevance theory seems potentially helpful for my argument, but I need to find work that explicitly addresses the link between the...

28 September 2015 8,493 3 View

Can anyone provide advice on how to model age captured in categories within SEM?

10 July 2015 4,972 15 View

How can I regenerate a Pt surface after polymerization?

I have a Pt crystal I would like to reuse. The Pt surface has been electrochemically grafted with a diazonium salt and subsequently polymerized with GMA. I'm thinking it should be possible to...

09 July 2015 5,149 2 View

Is there any recent work on literary language (or the nature thereof) or of "literariness" more generally?

Critics don't seem to be interested anymore in distinguishing between ordinary and literary language, but I'm wondering whether this is a crucial issue we need to return to.

06 October 2014 8,200 5 View

What is the best way to differentiate osteoclasts out of murine bone marrow cells?

Dear all, We have some problems with generating osteoclasts out of murine bone marrow cells. So I would like to ask in general: - Do you pretreat the cells before you culture them? - Which medium...

04 August 2014 9,354 4 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How Can I bring as much common elements in steel as possible to calculate stable phase(s) in OpenCalphad?

As I was tweaking step-by-step OpenCalphad6 Macros step1.ocm involving phase and thermal property calculation of a High-speed steel, I could not incorporate more than 6 elements in the steel...

01 March 2021 8,840 1 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View