I am trying by Prep TLC but not able get in enough quantity.
Use resins, silica, followed by C-18 or Sepadex LH-20 for isolation of EGCG.
non equi atomic high entropy alloy
05 March 2024 8,777 1 View
I synthesis new ligand, that ligand react with divalent metals it forms metal complex. l used maestro 9.0 for docking the protein with ligand but the problem was maestro 9.0 (not only that all the...
21 February 2024 2,485 1 View
Any vendor or Institute.
05 November 2023 3,030 0 View
Scratch Test
18 October 2023 4,063 6 View
Any solved example will be very helpful
30 September 2023 5,873 2 View
Entropy-VIKOR method is a MCDM technique
29 September 2023 8,233 2 View
Guide
03 September 2023 139 5 View
I have run for detection of IL-10 protein expression on day wise basis. Is this western blot correct?
18 August 2023 5,101 3 View
I want to find the thickness of Ti after the Diffusion of Ti in TiO2. So, which physics In COMSOL can help me to find the Diffusion and Thickness of Ti after the duffusion.
15 August 2023 4,033 2 View
I have already diposited Ti over Si3N4 and SiO2, but when i am deposition other material on Ti at high temparature around 1000-1500 °C, i did not found Ti over Si3N4 or SiO2. So, is it possible...
14 August 2023 953 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
Purification of gst tagged protein
25 July 2024 1,720 0 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Using INTRON's DNA-spin Plasmid DNA purification kit to extract plasmids from specific E.Coli strains, my team and I have encountered numerous plasmid dimerizations that lead to over-weighted...
23 July 2024 3,490 6 View
What is the heating temperature that I can use to deactivate enzymes in bacterial extract exopolysaccharide without loss of the bioactivity of EPS? Hi I am trying to make the purification (by TCA...
22 July 2024 5,334 6 View
Good day everyone, Trying to perform protein purification for the first time, gotta buy the whole equipment/reagents. Protein of interest 48 kDa (Antithrombin), expressed from his-tagged plasmid...
16 July 2024 3,692 6 View
Background: I am developing an RdRp assay for Dengue virus-3 and need to express and isolate the NS5 protein's RdRp domain (~101 kDa). My expression vector includes a GST tag followed by a 6x His...
13 July 2024 9,950 3 View
I was reading papers on plant secondary metabolites and their applications and many of the papers/books I read, have the point that plant-based medicines which are the result of application of...
08 July 2024 9,935 10 View