Piyush Chudasama

5 Questions 15 Answers 0 Followers

Questions related from Piyush Chudasama

What can be done to reduce the edge effect in cell culture multiplate (96 well plate)??

I am constantly getting variation in the data set. Need some suggestions if anybody can share their experience.

25 September 2014 742 6 View

How to induce apoptosis/oxidative stress by High glucose?

I want to induce apoptosis or oxidative stress by High glucose in HEK293 cells. But the media already contains 4.5g/L of glucose (25mM). How should i approach such experiments?

11 September 2014 9,550 5 View

How to use HP20 resin for fractionation?

I want to further fractionate my water fraction for isolating active molecule.

24 October 2013 9,624 11 View

My column cracked when I change my mobile phase solvent from non polar to nonpolar:polar solvent. Can anybody tell me the solution for this?

Column cracking due to trasition of non polar to polar solvent...

17 September 2013 6,691 15 View

Why does in vitro DNA protection assay or a fragmentation assay require plasmid? Can we use genomic DNA for the same purpose?

DNA damage assay of plant extracts

29 July 2013 1,828 2 View