Piyush Chudasama

9 Questions 18 Answers 0 Followers

Questions related from Piyush Chudasama

Is it necessary to synchronize my cell population before a DNA fragmentation assay or any apoptosis assay?

I am trying to develop DNA fragmentation in HEK293 cells since long but i am not able to develop positive control. I feel is there any need of synchronize the cells before treatment so that we can...

11 December 2014 1,983 6 View

Can anyone suggest why I am not able to observe comet tails in my positive control cells?

I am working on HEK293 cell line. The cells were treated with 5-FU for 24hr then I performed comet assay. Visibly many cells were dead but when I did comet assay nothing was observed (No comet)....

21 November 2014 5,976 8 View

What can be done to reduce the edge effect in cell culture multiplate (96 well plate)??

I am constantly getting variation in the data set. Need some suggestions if anybody can share their experience.

25 September 2014 783 6 View

How to induce apoptosis/oxidative stress by High glucose?

I want to induce apoptosis or oxidative stress by High glucose in HEK293 cells. But the media already contains 4.5g/L of glucose (25mM). How should i approach such experiments?

11 September 2014 9,596 5 View

How can I precipitate saponin from butanol fraction?

I have tried with diethyl ether but nothing happened. The same happened with using acetone. Can anybody can help regarding this?

16 July 2014 6,699 0 View

Can anyone share column chromatography based isolation of Epigallocatechin gallate?

I am trying by Prep TLC but not able get in enough quantity.

10 December 2013 9,446 1 View

How to use HP20 resin for fractionation?

I want to further fractionate my water fraction for isolating active molecule.

24 October 2013 9,676 11 View

My column cracked when I change my mobile phase solvent from non polar to nonpolar:polar solvent. Can anybody tell me the solution for this?

Column cracking due to trasition of non polar to polar solvent...

17 September 2013 6,744 15 View

Why does in vitro DNA protection assay or a fragmentation assay require plasmid? Can we use genomic DNA for the same purpose?

DNA damage assay of plant extracts

29 July 2013 1,879 2 View