We have a disruptor genie from scientific industries and regarding choosing the ideal bead size in order to lyse placental tissue, I would like to ask if anyone can recommend me sth?(I would like to extract proteins from my samples and have chosen standard RIPA buffer with proteinase inhibitor coctail. If you have any suggestions in this regard, I am also happy hear from you.)
When hitting the literature, I find anything between 1-7 mm bead size for mechanical "bead mill" tissue disruption.
Can you recommend stainless or glass beads?
Also how long would you perform the disruption? There I find anything between 1 and 5 min.
Many thanks in advance!
Hi Battuja Batbajar Dugershaw
In my knowledge, for any tissue samples less than 50 mg, one can go for disruption with 1.7-3.0 mm zirconium beads or small stainless steel balls.
Thank you.
Varsha
Dear Battuja Batbajar Dugershaw
Tungsten Carbide Beads (3 mm) can be used in protein extraction.
Also here I enclosed a protocol for protein extraction. I hope you will be successful in your experiment.
- Badges
- Science topic
- Similar topics
- Psychology
- Emotional Psychology
- Happiness