Anyone know good books for radioimmunoassay published in 2000 or later?
I've sent my plasmids for whole plasmid sequencing using the Oxford nanopore sequencing method. All of them were assembled as monomers, but in the reads (see attached pictures), there are even...
19 June 2024 1,280 5 View
Last week, I transformed home-prepared E. coli TOP10 cells. When checking the colonies, I noticed that some of them have yellow color. They did not grow on the randomly, but dependently on the...
16 June 2024 3,272 3 View
I'm trying to use reinforcement learning with live EEG measurements. However, just 2000 measurements/iterations take 16.6 minutes to measure and it seems I need at least 10 hours of live...
12 June 2024 2,884 3 View
A friend did PCR and then loaded it on agarose gel. All samples were prepared in the same way; the PCR was identical, with the same template, only primers differed, the same amount loaded on the...
04 June 2024 3,049 3 View
Hi! I am trying to do some boxplots in graphpad. I would like to use the 5-95 percentile boxplot option, but if I choose this option I do not know how can I also see the individual values of each...
17 April 2024 6,093 0 View
I did PCR of 6 amplicons and when I did agarose gel electrophoresis, they all appear consistently bigger than they should be (e.g. the amplicon should be 4000 bp, but is around band of 5000 bp...
09 April 2024 4,487 5 View
I was looking at a sediment sample from a "biosphere" that I´m creating and I came accross this little guy. I´m almost certain that it´s a freshwater diatom but would like to know the specific...
19 March 2024 1,763 10 View
I just found the Platinum SuperFi II DNA Polymerase, which should simplify PCR protocol as it allows uniform annealing temperature of 60°C, but it should also have very high fidelity; >300×...
17 March 2024 7,686 4 View
quisiera que me proporciones un sistema de gestion de calidad
15 March 2024 3,491 1 View
ME PUEDE PROPORCIONAR INFORMACION SOBRESistema de Gestión de Calidad Basado en la Norma ISO 9001:2015 para el Instituto de Educación Superior Tecnológico
15 March 2024 447 0 View
Hello, I am wondering if there is a good way to normalize my data. For background, I collect conditioned media from primary cells and then perform radioimmunoassay on them to determine the...
04 September 2023 2,588 5 View
I recently switched lot of a primary antibody used in a competitive RIA. I have performed a titration experiment and the new antibody now must be used a a 1:1M dilution to give the same maximum...
10 November 2020 5,133 0 View
As I read in papers, before doing the radioimmunoassay (RIA) to detect thyroid hormones such as T3, we should free the hormones from binding the carrier proteins like thyroxine-binding protein...
30 January 2019 2,366 1 View
Hello. We used Siemens count-a-coat cortisol RIA in a paediatric trial recently. Do any of you know whether this has been compared to (i) other immunologic assay methods or (ii) mass...
20 September 2017 2,080 0 View
08 September 2017 8,605 0 View
I prepared RIPA buffer to lysis my cells and went for protein assay, i added my protein samples to bradford right before measurging OD, but i didnt get homogenic mixture. i got a blue...
19 July 2015 6,193 5 View
anyone knows of comparative studies of elisa/eclia methods vs radioimmunoassay of plasma renin activity?
26 April 2015 3,628 1 View
03 April 2015 541 1 View
I am looking for the protocol of RIPA buffer in the internet and found many different kinds of RIPA buffer, some have NaF and some without, does anyone know the function of NaF in the RIPA...
12 March 2015 5,738 8 View
We frequently use the I-125 CORT RIA kit from MP biomedicals for mice and rats, but I have never measure ACTH from rat plasma before. I am wondering if anyone can recommend a good validated RIA...
01 February 2015 2,253 5 View
