Proper RP-HPLC workup following peptide synthesis?

Hello, I've recently synthesized a short (17aa) peptide via manual SPPS. I was instructed to submit a small pipette tip's worth of material onto a capillary column to obtain a rough estimate of...

12 January 2021 9,432 3 View

SPPS: peptide failure to elongate?

SPPS of a 16 aa peptide was performed on a 0.15 mmol scale. Resin gained a minimal amount of weight: starting weight of 224 mg and a final weight of 280.I believe my peptide failed to elongate but...

21 December 2020 9,428 11 View

No title

Greetings to all. I have some questions about setting up DFT calculations in Gaussian software (I am a beginner in these matters), and trying to calculate the energy of the first excited triplet...

06 December 2020 3,504 4 View

Problems with fluorescent bioconjugation of short peptide?

I have an 18aa azido-peptide conjugated at the N-terminus (cysteine). Synthesis and folding were validated through HPLC and mass spec. Multiple attempts were made with Dylight-phosphine conjugates...

02 August 2020 2,775 3 View

What is the experimental set up/or protocol to measure distribution of the light emitted?

Hello, Could anyone here please tell me the experimental set up, or the protocol to be able to measure the angular distribution of the light emitted (fD) by some OLED device? I have seen that...

10 February 2020 7,932 2 View

How to measure HRV-PRV agreement using Bland-Altman plots from Excel (step-by-step guide)?

Hi! I recently installed the Real Statistics complement for building Bland-Altman plots in Excel. However, I don't know how to use it in evaluating the agreement between HRV and PRV series. Could...

25 November 2019 3,736 3 View

What is the minimum number of PP intervals required for accurate and reliable extraction of Pulse Rate Variability (PRV) from PPG signals?

The minimum number of RR intervals required for reproduce several metrics derived from HRV (e.g., HF power) can be found in the literature (Richards et al., 2010). However, what about PPG signals...

02 June 2019 353 2 View

Diagnostic tool for mental health disorders in children and adolescence?

04 September 2018 7,983 6 View

Any fast publishing Scopus indexed journal ?

Who can help with a link to the a fast publishing scopus indexes journal. I am looking at a journal that will respond in like 3-5 weeks

19 August 2018 1,327 18 View

Metagenomics databases. Which one choose?

In bioinformatics databases play an important role in the results, whether chimera detection or OTUs assignment (16S metagenomics). In your experience, which databases have a better relation...

11 January 2018 858 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View

How to overcome convergence issues in concrete arch models?

Hi, I have modelled a three-span arch bridge with soil fill over along with pedestals. The FE modelling is done in LUSAS with HX8M solid elements (i.e. 3D solid modelling). The non-linearity is...

20 February 2021 1,346 2 View

Solid Phase Extraction of drug product containing nanoparticles ?

Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...

16 February 2021 1,389 2 View

Is there a paper that shows the effect of the thermal conductivity of solid electrolytes on lithium secondary batteries?

I want to know the effect of thermal conductivity characteristics of solid electrolytes on the all-solid battery, but I don't know which paper to refer to. Is there a paper on thermal behavior?

15 February 2021 8,806 2 View

How to match the XRD of a compound for which there is no JCPDS file ? Is there any other characterization to claim the synthesis of desired compound?

I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...

09 February 2021 921 3 View

COMSOL-lost contact problem-step study problem?

I'm trying to make a deep drawing model that contemplates a force on the blankholder and a sliding of the sheet under the same blankholder (a bit different from the 'die forming' model in the...

02 February 2021 7,599 1 View

Desalting using DSC- 18 SPE columns?

I am looking forward to remove excess of salt form my metal based porphyrin and trying DSC-18 columns for the same, can someone suggest me how how can one decide the polarity of the solvent to...

01 February 2021 9,076 1 View

What is the best way to improve the working potential window of a solid electrolyte?

My solid polymer electrolyte shows low bulk resistance show quite a low bulk resistance. However, the potential window (obtained from LVS) is very small. Please how can I improve the operating...

31 January 2021 3,340 9 View