Can anyone recommend a protocol to isolation neutrophils from mouse liver?
Thanks
Ilyas
Hi, IIyas, neutrophils in the liver are the same neutrophils that are in the circulation.
Do you mean liver macrophages?
Colagenase treatment and then dextran+ficoll protocols. That's used for tissue cells. However, you must check cell activation after that.
Try gentle homogenization by hand w tweezers, filter cells from debris (100nm). Plate in FCS pretreated Petri dish, 37°C 30 min, wash with warm medium, check under scope, remove PMN with cold medium GENTLY.
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