Can anyone recommend a protocol for isolation of neutrophils from mouse liver?

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Steingrimur Stefansson

Hi, IIyas, neutrophils in the liver are the same neutrophils that are in the circulation.

Do you mean liver macrophages?

Juan Carlos Hernández

Colagenase treatment and then dextran+ficoll protocols. That's used for tissue cells. However, you must check cell activation after that.

Hector Pons

Try gentle homogenization by hand w tweezers, filter cells from debris (100nm). Plate in FCS pretreated Petri dish, 37°C 30 min, wash with warm medium, check under scope, remove PMN with cold medium GENTLY.