Hello

Try with sigma , novusbio , biossusa , biocompare , R&D system , also check out of the assays which can be used with the your antibodies in datasheet ( see the img)

Good Luck

http://www.biocompare.com/pfu/110447/soids/42359/Antibodies/HSL

http://www.sigmaaldrich.com/catalog/product/sigma/sab4501762?lang=en®ion=SA

http://www.novusbio.com/Hormone-sensitive-Lipase-HSL-Antibody_NB110-37253.html

http://biossusa.com/store/products/primary-antibodies/bs-0455r.html

Hi,

we always use the HSL #4107 CellSignaling antibody. Works pretty fine in tissues and cells.

Thanks for the answers Saleh and Thomas.

We also use CellSignaling HSL #4107 for WB, it works the best. You may want to consider also measuring phosphorylated HSL if doing a lipolysis assay. CST has a great anti-pHSL for Ser660 residue #4126.

Thank you for the answer John, but the antibody you mention works with rat tissue?

We have never tried it on rat but rather on mouse and --our lab's species of choice-- Siberian hamster both antibodies work perfectly. Homology for HSL is very high between mice and rat so worth a try indeed.

Ok! Thanks again!

I've heard that the Cell Signaling HSL antibody mentioned above works well.

Hi all

I just tried the Cell signaling pHSL and HSL antibodies (#4107 and #4126) in rat adipose tissue (perirenal and subcutaneous) and they work perfect.

Thanks to all of you for the help

Iñaki

Does anyone know a good antibody for pHSLser563 (equivalent to human pHSLser552) for IHC. I could only find one by Thermo Fisher but that is for ICC, not IHC.

Many thanks

SF