hi

Microscopic control of the interaction between NK-92 and primary NB cells

Primary NB cells were seeded at low density on 20 mm chamber slides (Nunc, Langenselbold, Germany) and grown for 24 hrs. Then adherent NB cells were co-cultured with gene-modified NK-92-scFv(ch14.18)-ζ or parental NK-92 cells for 4 hrs at an E:T ratio of 5:1. NK-92 cells were identified with CD45-specific antibody followed by FITC-conjugated secondary antibody. CAR expressing NK-92 cells were detected with mAb 9E10 followed by PE-conjugated secondary antibody. All cells were counter-stained with DAPI. Fluorescence microscopy was performed with an Olympus IX71 microscope (Olympus, Hamburg, Germany), and microscopic images were acquired with a charge-coupled device camera.

good luck

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822932/

https://www.researchgate.net/post/troubleshooting_NK92mi_cells_proliferation_when_and_why_they_start_dying_after_being_thawed

Hi,

Thanks a lot for your suggestions. I will try these ones. 

Regards