Can anyone please share me the protocol for making single cell suspension from brain tissue

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Adam Stewart

Hi,

I'm just about to start optimising a method for tissue disassociation myself so I can't give a definitive SOP but i have found this list of references a very useful starting place:

http://www.worthington-biochem.com/tissuedissociation/Tumor.html#11

Hope this helps.

Qadir Alam

Adam Stewart Thanks alot