Actually I want to know how does it work in comparison with electrochemical cell. What happens to working, reference and counter electrodes? How electrolyte participate in the procedure?

I've been working on my thesis using screen printed carbon electrodes for about 2 years. Question is quite broad but I'll do my best.

Screen printed electrodes (SPEs) are integrated systems which combines working, counter and reference electrodes on insulating substrate. SPEs work in an electrochemical cell which contains desired solutions, for example redox couples or monomers for electropolymerisation. There are some kinds of SPEs, carbon and platinum are worked on mostly but screen printed gold electrodes are also available. I've been working with screen printed carbon electrodes produced by Pine Instruments but given a try to platinum ones. There are also specialized designs that bring up to 8 electrodes -if I remember the figure correct- together for 8 simultaneous experiment.

Carbon electrodes are usually disposable but platinum electrodes can be cleaned electrochemically and used for several times. Manufacturers generally present electrochemical properties of electrodes on their websites, for example cyclic voltammetry plots in an electrochemical cell which contains redox couples.

If there is any further questions, feel free to ask please.

Thank you and good days.

Thank you dear Mustafa. Actually I need to know how electron transfer mechanism happens via the these three electrodes. How electrolyte acts in the chemical reaction. 

I was wondering if you could please inform me about it.

Dear Artur,

I am working on gold electrodes at room temperature. I want to know electron transfer mechanism of three electrode SPEs happen and how electrolyte is important. Does electrolyte make chemical reaction with electrodes? What is the substitute of electrolyte in electrochemical cell while working on three electrode SPEs?

Dear Artur,

yes I am working with gold screen printed electrodes.

You can find a schematic sketch in the attached file.

I want to functionalize my working electrode to detect a biosensor reaction.

Hence, electrolyte does not react with electrodes.

In a biosensor reaction where redox happens and how electrolyte is engaged?

Dear Ali Akbar,

For your last question (sorry I didn't check questions), redox reaction occurs between biological agent of biosensor and redox couple in solution. If you have an enzyme electrode, catalitic activity may need or release electrons that stimulate electron exchange between redox couple. We also use redox couples when we work on immunosensors in which no electron exchange happens for the biological agent. In this case, the potential of electrochemical cell drives the electron exchange between redox couple.

You can find much more information about this phenomena in any thesis work related to electrochemical sensors.