Do you know Psychometric Test or questionnaires specifically designed to evaluate tennis players?

28 September 2020 6,850 7 View

Why does my PCR not show whether my mice are homozygous or heterozygous for a floxed gene?

So, I am trying to determine whether my mice are homozygous or heterozygous for a floxed gene. I ordered the primers specified by the company which developed the mice and they amplify a 301 bp...

10 March 2020 9,740 4 View

In the Raman spectra, why in some cases band D moves to the left and in other cases moves to the right?

I am analyzing carbon nanotubes by nanocasting (MNC), using SBA15 as a hard template. Composite C-SBA15 is the intermediate material with the carbon precursor within the SBA15 channels. In the...

14 May 2018 5,813 4 View

What are possible substitutes for P2O5 as a desiccant?

I've been using phosphorus pentoxide as the desiccant while drying my enzymes; however, our stockroom has now run out of the chemical. What are other chemicals that can be used as desiccants that...

12 January 2018 8,072 4 View

How to deal with the ndvi range variation from landsat 7 and landsat 8?

I try to study the vegetation change using NDVI from landsat 7 and landsat 8 wherein the maximum NDVI value for NDVI from landsat 7 is 0.8 whereas for the NDVI from Landsat 8 is only 0.6 but...

21 October 2016 1,158 9 View

Why a PCR is inhibited by DNA extracted from soil samples?

The PCR work very well with Parasite DNA, but, when the Parasite DNA is added to the soil samples and the DNA is extracted from contaminated soil samples, to used in the same PCR, the PCR is...

29 May 2016 5,065 5 View

How do I Improve the stability of membrane protein extracts from M.haemolytica?

I am currently working with Mannheimia haemolytica. We make a membrane protein extraction with sodium salycilate (SS) and then we keep it with the SS at the same concentration (1 M). When kept at...

29 July 2015 7,081 1 View

What are the best markers to distinguish liver cells?

I want to know which liver cells take up fluorophore labeled spherical nucleic acid nanoparticles using either FACS or IHC. I'm interested in specific markers for Kupffer cells, stellate cells,...

07 April 2014 9,366 4 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View