I have isolated this fungus from Soil (rhizosphere) of Tomato plant. The fungi producing this type of growth pattern like a rose. While staining with lactophenol cotton blue it long hyphi observed without any typical spore formation.
Plating was done in Oat Meal Agar and incubation temperature at 160C for 12days.
It looks to me that you may be dealing with Phythopthora root rot. That is not a positive identification but the mycelium looks consistent and needs to verified. To identify on morphology you will need to stimulate the Sporania and the release of zoospores from it. You also could use DNA PCR blast to get positive identification. Phytophthora parasitica is a root rot pathogen fairly common on tomatoes in some areas.
Why dont you going for 18s rRNA sequencing, i thing it will be helpful in identifying fungi rather that just predictions
Dear Priyanka,
This rose-like growth of colony is typical for Mortierella species. The genus comprises litter and soil saprotrophs, but it is needful to obtain sporulation (typical for Zygomycetes) or to make DNA analysis for clearing the problem,
Best regards,
Elena
Dear Priyanka Roy
I agree with Paul Reed Hepperly .
However, its better that you culture this isolate on PDA. By the way, you can use hemp seed for sporangia production and the release of zoospores from them. I cannot see the hyphae of your isolate clearly. If it has not septum so close to Oomycetes. Finally, you can check it by molecular methods.
Regards
Hamid
Agree with Dr. Elena that sporulation is required for preliminary identification.
Agree with Dr. Elena. This genus is quite common saprophyte and rose like mycelium is a good identification point
83
I think, it is facultative saprophyte You need to induce of sporulation, the host may be insect or nematode
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