How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Cox regression baseline hazard?

Dear colleagues, I would like to make a mathematical equation of Cox regression model, however I am stuck with baseline hazard. I clearly understand interpretation of coefficients, but I can't...

24 February 2021 6,207 3 View

How to calculate the electric field in the rotating frame (the question in the general relativity)?

In the classical electrodynamics, there is one problem - to find the EM fields created by the charge moving with the constant velocity in the circular orbit or in the helical path (free electrons...

12 January 2021 3,689 1 View

Do you know anything about Elena Bokus, the early 20th Century Architect / Architectural Designer?

The available research of early 20th century history of architecture in North Macedonia, suggests that probably the first female architect / architectural designer was Elena Bokus (born in 1893...

23 December 2020 2,039 2 View

Can you recommend a short and free Deep Learning course that is sufficient to enable implementation on Java?

It would be best if the material is available on the web, a pdf version is also possible. If you still have knowledge of a good video course, please share.

29 November 2020 4,769 2 View

Why the Helmholtz decomposition theorem is not applicable to the electrodynamics?

My question is caused by one curious fact of the electrodynamics. It is commonly accepted opinion that the electromagnetic fields can at least formally be decomposed onto the rotational and...

10 September 2020 8,668 3 View

What solutions do you suggest for molecular field visualization/analysis?

Hello, I wonder what options exist these days for visualizing "molecular field" type data. That is, a 3D scalar field represented by translucent volume rendering or isosurfaces, and overlaid on a...

12 June 2020 861 2 View

COS7 cell transfection - do you have a good protocol?

Dear all, lately we ran into trouble with COS7 cell transfection, we get low transfection rate with the protocol we use. Do you have a good transfection protocol? Preferably Calcium transfection...

23 May 2020 842 2 View

How to choose collective variables for ligand binding to protein?

Hello! What is the recommended way to choose collective variables / reaction coordinates to study ligand binding to a protein (e.g., for PMF free energy profiling)? I often see the advice like "Do...

10 March 2020 1,878 4 View

How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for Metabarcoding?

I'm working on pollen DNA metabarcoding to identify the floral composition of honey. How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for the...

28 December 2020 9,132 1 View

Can anyone help in doing SEM of pollen?

Is there any institute in Kerala were v can do SEM studies.

11 November 2020 5,001 3 View

I need info on particular Convolvulaceae pollen, can you help?

Dear colleagues in palynology, paleobotany, and botany I desperately need information/papers on the pollen morphology of the following Convolvulaceae genera: Astripomoea, Calycobolus,...

14 October 2020 8,216 10 View

What is the best method for collecting and preparing pollen from insects stored in 70% ethanol, for visual identification?

Hello, I would like to visually identify and quantify the pollen morphotypes carried by insects which have been stored in 70% ethanol. The insects are Diptera (mainly Syrphids), and Hymenoptera...

20 August 2020 8,859 3 View

Is it possible to remove pollen from museum bee specimens?

I have recently extracted genomic DNA from some museum bees for sequencing, and from first look there appears to be some pollen DNA in the extract. I've had a Google but can't find anything...

25 May 2020 8,423 2 View

Can adult butterfly or moth feed on solid food, such as pollen?

Dear All, Can adult butterfly or moth feed on solid food, such as pollen? It is known that nectar mainly contains sugar but very low level proteins, lipids. If a butterfly only feeds on nectar,...

29 March 2020 6,211 3 View

Species that contains the grass allergen Phl p5?

Does anyone know in which species the grass allergen Phl p5 occurs? It was originally isolated from the grass species Phleum pratense so it must at least occur in that species. It stand to reason...

15 March 2020 8,703 4 View

Chemical laboratories in Tunisia ?

01 February 2020 5,381 1 View

How different do fossilised and fresh pollen look under the microscope?

I was wondering whether fossilised pollen looks very different from normal pollen when you use bright field microscopy? In particular, do the following change significantly: Morphology...

29 November 2019 1,269 9 View

When doing Scanning Electron Microscopy of pollen samples from basal angiosperm group, is it necessary to do acetolysis?

1. Will there be significant differences in SEM images of acetolysed pollen and pollen fixed in 70% EtOH? 2. This is to study the micro-morphology of pollen structure and ornamentation for...

10 November 2019 5,022 2 View