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Does anyone have a protocol for measuring ethanol and acetaldehyde in plant tissues?

I am planning to measure the ethanol and acetaldehyde contents in the leaves and crowns of turfgrass that were subjected to varying durations of ice encasement. Does anyone have a protocol for...

07 April 2024 2,755 1 View

Why ammonia decomposition not known on Mn based catalyst?

Why Mn catalysts are not in literature for decomposition of ammonia. There is a lot of ammonia degradation on its nitrides but not on Mn oxides. Why it is so?

20 January 2024 3,536 6 View

In the case of Metal-air battery (DFT approch), how can I find Entropy and Zero point energy?

Equation of Gibbs free energy G=∆E-ZPE-T∆S How finds the entropy and ZPE

08 January 2024 9,593 0 View

Has anyone used any dye for viability assay in pants?

Has anyone used Sytox Blue, Propidium Iodide, Trypan Blue, Evans Blue, TTC, Alamar Blue, Neutral Red, and FDA dyes to determine the viability of crown and roots in perennial ryegrass and/or plant?...

24 May 2023 4,322 1 View

How to anneal CZTS deposited on Molybdenum?

I am sputter depositing a CZTS layer on molybdenum back electrode on a SLG substrate. The sample turns black and not usable for the soalr cell application when I anneal it at 450 C, in Argon...

19 April 2023 2,063 2 View

Any Physiotherapy tratment protocol available for different stages of AVN HIP..?

Can any one discuss about Physiotherapy tratment protocol for different stages of AVN Hip.

26 February 2023 2,313 2 View

Why does MCF-7 cells grow in colonies?

I am working with breast cancer cells. It's a general fact that each cell lines have its own morphological appearance. but I am looking for the mechanism responsible for the morphological...

20 February 2023 2,607 2 View

What statistical analysis can be applied to a randomised clinical trial with 4 interventiion arms ?

Hi Sir/Mam, What statistical analysis can be applied to a randomised clinical trial with 4 intervention arms ? I have collected data for baseline, 4 weeks and 8 weeks respectively for four...

12 December 2022 3,227 12 View

How modelled compression only spring in ANSYS WorkBench?

I have modelled solid slab and I have assigned bottom face of slab as Elastic support but that elastic support is needed to be modelled for compression only spring.

16 October 2022 4,365 3 View

Hello there, how can i coat thin layer of Gold on the surface of PDMS films using bright electroless gold plating solution?

I tried many times following drop-casting of electroless gold plating solution, but after wash, there is no more conductive layer. I also tried making the surface of PDMS polar using PDA. Can you...

17 August 2022 2,746 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

Interpreting ATR-FTIR Spectra for Microplastics Research?

Hello everyone, I am currently pursuing a PhD in Environmental Engineering with a focus on microplastic studies. My background is in Civil Engineering, and I am relatively new to working with...

18 July 2024 7,258 3 View