What are the benefits exactly of synthetic intron (IVS) and internal ribosome entry site (IRES) on a plasmid?

Hello everybody! so I have a plasmid which has a synthetic intron (IVS) & an internal ribosome entry site (IRES), and I'm thinking to remove them as I'm cloning due to some matters related to...

26 January 2021 7,645 2 View

How we can calculated LOAEL and NOAEL for toxic pathological dose ?

how we can calculated LOAEL and NOAEL for toxic pathological dose

02 January 2021 1,175 2 View

What are the new methods of staining??

what are the new methods of staining??

25 December 2020 4,030 3 View

Hi, I want to run simulation with YASARA for different part of my protein separately but I dont know how to run?

I am working on enzyme engineering and want to run the simulation for my enzyme the open and closed conformation to see the effect of the mutation on my enzyme as its located on the lid. However,...

10 May 2020 2,625 1 View

I am working on COVID19 dataset??

I am working on COVID19 dataset, but, When I tried to convert the data.xlsx file to CSV file, I got an error. Also, i got an error when I upload the CSV file to WEKA. I tried to solve the problem...

05 May 2020 4,879 2 View

How can i define the USRP N210 on pc under windows 10?

How can i define the USRP N210 on pc under windows 10 and i used gnu radio 3.8.. i set the configuration and i make the ping and recived reply successfuly but i cannot define the device and an...

21 January 2020 1,442 2 View

What is the number of measurement required in Circulant and Toeplitz sensing matrix in general in term of N an M in compressive sensing?

what is the number of measurement M (the number of measurement vector that result from multiplication of sparse vector N with sensing matrix (NxM)) required when using Circulant and Toeplitz...

16 January 2020 9,666 2 View

Does Tobramycin have an amorphous structure?

I've been analyzing the crystallinity of Tobramycin by the XRD, it is supposed to have a crystalline structure, but when analyzed, it showed an amorphous structure

25 November 2019 5,145 1 View

I've been trying to test the antimicrobial activity of zinc oxide nanoparticles against P.aeruginosa. They exhibited no activity. Any one knows why?

the nanoparticles were purchased from sigma and they have a size range (35-50 nm). i tried to dissolve it in DMSO ( 5% of the nanoparticle solution) and i also tried to sonicate it for 30...

24 August 2019 9,678 10 View

Combustion Engineering second edition by Kenneth W. Ragland, Kenneth M. Bryden?

I looked for this book literally everywhere but I can't find it, dose anyone have a PDF copy of this book because I really need it...

01 April 2019 6,305 3 View

Can anyone tell me the name of these parasites which I got from a marine fish?

I got these parasites from eye and intestine of a marine fish. Thank you.

09 December 2020 7,752 2 View

How to estimate the effect of nematodes on some varieties of wheat?what are the correlation possible to do ?

Hi , I carried out a test on the behavior of some varieties of durum wheat with respect to the CCNs, two successive years, in pots, and I inoculated with the same number of cysts (Pi), at the end...

05 December 2020 7,187 3 View

How to fetch data of a .sslist file?

We are using Mauve software for alignment of genomes of nematodes having genomic size approximately 100 Mb. One of the output file generated using this software has extension .sslist file. Unable...

24 September 2020 7,520 1 View

Someone recommend to use CFSE to stain parasites as Trypanosoma or Leishmania species to detect cells infection?

I have seen that some groups use CSFE to stain some parasite species before infect cells to detect percetage of infected cells. Could I use CSFE to stain Trypanosoma cruzi and measure infection in...

15 August 2020 1,898 2 View

What is the best method to get a good appearance or view or photo for Leishmania parasite????

i need an easiest technique for Fluorescent stain used with Fluorescent Microscope .

14 July 2020 3,551 3 View

How can I hide nodes with posterior probability under 0.5 in BEAST phylogenetic tree visualization?

06 July 2020 1,376 6 View

Could someone recommend me a journal for the publication of a short note discussing the validity of some parasitic nematode species?

These species were described in a PhD thesis and all of them but one remain unpublished.

06 June 2020 4,786 3 View

What is the (average) DNA yield (ng) from one single nematode?

Do you know where I can found published information about DNA quantity (in ng) from one single nematode? Any publications/books to suggest me? I Know it seems a simple and basic information, but...

31 March 2020 6,710 1 View

Why second stage juveniles are used for DNA extraction from nematode for identification purpose? Can I use female or male for the same purpose?

I am working on rice root knot nematode. For identification purpose mostly second stage juveniles are used. I wonder why females or males are not used commonly for the same purpose? I am using...

26 March 2020 7,419 3 View

Where can I get a reliable list of Wieser's (1953) nematode feeding type classification?

Numerous articles cite it but nobody seems to provide an actual list of the taxa and which of teh four groups they are assigned to.

21 March 2020 5,990 5 View